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2DE map comparison and alignment of bovine milk proteins using in-gel rehydration or paper-bridge loading. 600 μg of non-fat dry milk proteins were in-gel loaded using 3–10 IPG strips (A) or paper-bridge loaded using 6–11 IPG strips with (C) or without electrode wicks (D) during focalization. In a second time, paper-bridge previously used with 6–11 IPG strip was applied with 4–7 IPG strips (B) to focalize remaining proteins.
