Figure 3. Immunohistochemical staining of onion root sections.

(A) Vertical slice of an onion root. The magenta and green boxes indicate columella and cortex, respectively. Anti-AfiCENH3, anti-α-tubulin and DAPI signals are indicated in red, green and blue, respectively. (B) and (C) are close-up images of the magenta and green boxes in (A), respectively. (D) Horizontal slice of an onion root. Anti-AfiCENH3, anti-α-tubulin and DAPI signals are indicated in red, green and blue, respectively. The magenta and green boxes indicate columella and cortex, respectively. (E) and (F) are close-up images of the magenta and green boxes in (D), respectively. (G) A 3D image constructed from a set of Z-stack pictures. Anti-AfiCENH3, anti-α-tubulin and DAPI signals are indicated in red, green and grey, respectively. Scale bar, 100 µm.