Figure 4. Comparison of acetic orcein staining, immunostaining and immunohistochemical staining in mitotic cells of onion.

Chromosome pictures of acetic-orcein-staining (A–E), general immunostaining (F–J), and immunostaining of sections (K–P). Red, green and gray signals in F∼P are signals of anti-AfiCENH3 antibody, anti-α-tubulin antibody and DAPI, respectively. Scale bar, 10 µm. (G–V) A set of diagrams based on the results in K–P.