Table 1.
Overview of the structural proteins identified by ESI-MS/MS
| Protein number | Protein name | Protein MW (Da) | Max. No. of unique spectra | Maxi. sequence coverage (%) | Slice in which most abundant | Remarks |
|---|---|---|---|---|---|---|
| gp2 |
Conserved hypothetical protein |
81,838 |
28 |
53.9% |
5 |
|
| gp3 |
Putative DNA circulation protein |
43,673 |
6 |
22.6% |
9 |
|
| gp6 |
Conserved hypothetical protein |
19,283 |
6 |
43.3% |
20 |
|
| gp7 |
Baseplate protein; phage P2 GpJ homolog |
43,379 |
11 |
43.9% |
9 |
|
| gp8 |
Hypothetical protein |
31,431 |
5 |
27.3% |
13 |
|
| gp9 |
Hypothetical protein |
31,400 |
4 |
23.2% |
13 -14 |
|
| gp102 |
Putative tail fibre |
61,504 |
6 |
17.7% |
4 |
|
| gp103 |
Hypothetical protein |
14,237 |
4 |
44.7% |
22 |
|
| gp106 |
Hypothetical protein |
14,221 |
7 |
51.7% |
23 |
|
| gp107 |
Hypothetical protein |
52,046 |
18 |
36.9% |
1 |
|
| gp108 |
Hypothetical protein |
37,504 |
3 |
13.5% |
11 |
|
| gp111 |
Hypothetical protein |
4,295 |
2 |
45.2% |
25 |
|
| gp113 |
Portal protein |
45,459 |
10 |
34.2% |
9 |
|
| gp114 |
prohead protease |
28,590 |
2 |
10.5% |
18 |
|
| gp115 |
Major capsid protein |
52,513 |
19 |
46.5% |
11 -13 - 14 |
only 'C-terminal' sequence coverage |
| gp116 |
Hypothetical protein |
14,261 |
3 |
37.9% |
20 - 21 - 22 |
|
| gp117A |
Hypothetical protein |
3,205 |
4 |
85.7% |
25 |
|
| gp118 |
Hypothetical protein |
24,820 |
9 |
44.3% |
14 |
|
| gp119 |
Hypothetical protein |
22,041 |
9 |
84.7% |
17 |
|
| gp121 |
Hypothetical protein |
15,986 |
3 |
35.0% |
19 |
|
| gp122 |
Hypothetical protein |
23,047 |
6 |
20.5% |
15 |
|
| gp124 |
Hypothetical protein |
20,253 |
1 |
8.4% |
18 |
protein identification probability of 87.70% |
| gp126 |
Tail sheath protein |
54,066 |
21 |
50.5% |
7 |
|
| gp127 | Hypothetical protein | 14,475 | 5 | 50.7% | 18 |
For every detected protein the protein name, the predicted molecular weight (Da), the maximum number of unique spectra and sequence coverage (%) is listed. Moreover, the gel slice in which those maximums were observed is given, as well as some additional remarks.