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. 2012 Oct 30;13:33. doi: 10.1186/1471-2199-13-33

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Copyright ©2012 Killen et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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RecG has no effect on gene cluster instability in cells with normal BLM. Southern blotting detection of the rDNA clusters in BLM+ cells stably expressing higher levels of co-translated RecG/EGFP, “BLM+: RecG 2a”. Cluster lengths resolved up to 1 Mb are shown. Cluster lengths are calibrated by S. cerevisiae chromosome size markers. Open star is the 1 Mb resolution limit of the gel: all clusters larger than 1 Mb co-localize in this region. A clonal cell population is shown in the far left lane of the gel. A collection of subclonal populations each derived from a single cell of this clonal population are shown to the right. Plum circles: relocated major band clusters. Blue braces: former location of major band clusters from the parental clone.