Table 1.
SCE levels and EGFP expression
| Cells | SCE/Chr | EGFP | % EGFP+ | HPF | Xomes |
|---|---|---|---|---|---|
| HeLa |
0.09 |
1.0 |
0 |
21 |
605 |
| HeLa: RecG fuse |
0.08 |
42.6 |
98 |
32 |
835 |
|
BLM+ |
0.10 |
1.0 |
0 |
22 |
863 |
|
BLM+: RecG 2a |
0.10 |
49.3 |
100 |
30 |
999 |
|
BLM-: cDNA |
0.28 |
1.0 |
0 |
26 |
1625 |
|
BLM-: RecG 2a high |
0.33 |
148 |
93 |
33 |
1861 |
|
BLM-: RecG fuse high |
0.34 |
29.8 |
87 |
26 |
1149 |
|
BLM-: RecG 2a low |
0.68 |
12.4 |
92 |
36 |
2017 |
|
BLM-: EGFP |
1.02 |
27.3 |
57 |
48 |
3013 |
|
BLM-: empty vector |
1.05 |
1.0 |
0 |
25 |
1354 |
|
BLM- |
0.98 |
1.0 |
0 |
16 |
883 |
|
BLM- FC: RecG fuse |
0.70 |
6.4 |
100 |
55 |
1981 |
|
BLM- FC |
0.91 |
1.0 |
0 |
49 |
2077 |
|
BLM-: RecG fuse med |
0.43 |
30.6 |
87 |
29 |
1925 |
| BLM-: lost RecG fuse | 0.86 | 8.96 | 1 | 25 | 1316 |
Cells: The stable cell line being investigated.
SCE/Chr: Median number of sister chromatid exchanges observed per chromosome (sister chromatid pair).
EGFP: Relative levels of fluorescence for cells expressing RecG and/or EGFP, relative to cells not expressing EGFP.
% EGFP+: The fraction of cells in a population with above-background green fluorescence due to expression of EGFP.
HPF: The number of high powered microscope fields scored.
Xomes: The total number of sister chromatid pairs scored for exchanges.