Table 1.
Methylation status of miR CpG islands in gastric mucosa samples with different pathological changes in GC and non-cancerous control patients
| miR CpG islands | Group of gastric samples |
miR -Methylation |
||||||
|---|---|---|---|---|---|---|---|---|
|
Positive rate |
Proportion (%) of methylated miR in the miR methylation-positive samples |
|||||||
| Positive rate (%) | χ2-value | Trend-test (P-value) | Median[25% ~ 75%] | Mean±SD | t/F/χ 2-value | P-value | ||
|
miR-9-1 |
Normal |
1/13 (7.7) |
27.598 |
<0.001 a |
NA |
|
|
NA |
| |
Gastritis |
1/35 (2.9) |
|
|
NA |
|
|
|
| |
SM |
9/28 (32.1) |
|
|
17 [13–30] |
20 ± 4 |
t = −3.039 |
0.005 g |
| |
GC |
18/28 (64.3) |
|
|
29 [19–40] |
32 ± 4 |
|
|
|
miR-9-3 |
Normal |
6/13 (46.2) |
23.389 |
<0.001 a |
38 [31–59] |
43 ± 6 |
F = 1.639 |
0.189 h |
| |
Gastritis |
15/37 (40.5) |
|
|
43 [36–48] |
42 ± 2 |
|
|
| |
SM |
26/28 (92.9) |
|
|
38 [26–44] |
36 ± 2 |
|
|
| |
GC |
26/28 (92.9) |
|
|
37 [26–43] |
36 ± 2 |
|
|
|
miR-137 |
Normal |
5/13 (38.5) |
18.626 |
<0.001 a |
10 [4–41] |
|
χ2 = 8.065 |
0.045 d |
| |
Gastritis |
24/38 (63.2) |
|
|
19 [7–36] |
|
|
|
| |
SM |
25/26 (96.4) |
|
|
15 [7–40] |
21 ± 3g |
|
|
| |
GC |
24/26 (92.3) |
|
|
36 [20–51] |
37 ± 4 |
|
|
|
miR-34b |
Normal |
6/13 (46.2) |
6.947 |
0.008 a |
13 [7–19] |
|
χ2 = 0.658 |
0.883 d |
| |
Gastritis |
13/36 (36.1) |
|
|
11 [3–20] |
|
|
|
| |
SM |
22/28 (78.6) |
|
|
11 [4–18] |
|
|
|
| |
GC |
19/28 (67.9) |
|
|
10 [5–32] |
|
|
|
|
miR-200b |
Normal |
13/13 (100) |
0.486 |
0.922 f |
54 [52–83] |
|
χ2 = 17.883 |
<0.001 d |
| |
Gastritis |
35/36 (97.2) |
|
|
52 [46–100]e |
|
|
|
| |
SM |
27/28 (96.4) |
|
|
44 [40–47] |
|
|
|
| |
GC |
27/28 (96.4) |
|
|
48 [40–53] |
|
|
|
|
miR-210 |
Normal |
2/13 (15.4) |
11.908 |
0.001 a |
NA |
|
|
NA |
| |
Gastritis |
13/38 (34.2) |
|
|
7 [5–10] |
|
|
|
| |
SM |
22/27 (81.5) |
|
|
11 [5–22] |
|
|
|
| |
GC |
16/27 (59.3) |
|
|
9 [4–16] |
|
|
|
|
miR-193b |
Normal |
0/13 |
7.045 |
0.008 b |
NA |
|
|
NA |
| |
Gastritis |
2/37 (5.4) |
|
|
NA |
|
|
|
| |
SM |
7/28 (25.0) |
|
|
5 [4–14] |
|
|
|
| |
GC |
4/28 (14.3) |
|
|
5 [2–12] |
|
|
|
|
miR-203 |
Normal |
5/13 (38.5) |
5.617 |
0.018 b |
32 [29–75] |
|
χ2 = 2.957 |
0.398 d |
| |
Gastritis |
20/38 (52.6) |
|
|
31 [26–36] |
|
|
|
| |
SM |
21/28 (75.0) |
|
|
34 [30–36] |
|
|
|
| |
GC |
11/28 (39.3)c |
|
|
32 [29–35] |
|
|
|
|
miR-375 |
Normal |
0/13 |
12.266 |
<0.001 b |
NA |
|
|
NA |
| |
Gastritis |
13/38 (34.2) |
|
|
3 [3–4]e |
|
|
|
| |
SM |
16/28 (57.1) |
|
|
7 [4–14] |
|
|
|
| GC | 8/28 (28.6) c | 17 [8–21] | ||||||
a,Trend test, among Normal, Gastritis, SM and GC samples; b,Trend-test, among Normal, Gastritis, and SM samples; c, Pearson’s Chi-square test, GC versus SM: miR-203, χ2 = 7.292, P = 0.007; miR-375, χ2 = 4.667, P = 0.031; d, Kruskal-Wallis H-test; e, Mann–Whitney U-test: Gastritis versus SM, miR-200b,U = 230.000, P = 0.001; Gastritis versus GC, miR-375, U = 46.000, P = 0.011; SM versus GC, miR-137, U = 170.000, P = 0.009; f, Pearson’s Chi-square test, among Normal, Gastritis, SM and GC samples; g. Paired t-test: SM versus GC, miR-137, t = −2.652, P = 0.014; h. One-way ANOVA; NA, not available.