Table 2. Primers used for gene cloning, checking morpholino efficacy and site-directed mutagenesis.

	Forward Primer (5′–>3′)	Reverse Primer (5′–>3′)
clone appa cDNA	AGAAGCATGCGGTCGAGGGA	GTGACGGTGCTCCATCAGTTG
clone appb cDNA	CAGCCATGGGTATAGACCGCA	TTAGTTCTGCATTTGCTCAAAGA
clone prp1 cDNA	CAAAATGGGGGAGTTATGCAAAC	CATTAAGTGGTACTAAAAAGCATAG
clone prp2 cDNA	ATGGGTCGCTTAACAATACTATTG	TGAGAATGTCAGTGTAGAAGGGA
clone sho1 cDNA	ATCCAGAATGAACAGGGCAGTC	CTCAAGGGGCAAAGTGCATCAT
Confirm appa MO efficacy	GAGCTCGAGGATGAACACTA	ACAGCGGCGCTCTCAGACT
Confirm appb MO efficacy	AGCCTGTCAGCATCCAGAAC	CACCGTCTTCATCGTTGTCC
Create appa∧S3X;E5X	CTAGAAGCATGCGGTAGAGGTAGCTCTTCATATTAC	GTAATATGAAGAGCTACCTCTACCGCATGCTTCTAG
Create appa∧14_15insT	CATGCGGTCGAGGGATGCTCTTCATATTAC	GTAATATGAAGAGCATCCCTCGACCGCATG
Create appb∧M3X;V7X	GCTCAGCCATGGGTTAAGACCGCACG TGATTCCTGCTTTTAATG	CATTAAAAGCAGGAATCACGTGCGGTCT TAACCCATGGCTGAGC
Create appb∧del7A	GCTCAGCCATGGGTTAGACCGCACGG	CCGTGCGGTCTAACCCATGGCTGAGC