Table 1.
Validation parameters for first and ratio first derivative spectroscopic methods
|
Parameters assessed |
Method D1* |
Method RD1** |
||
|---|---|---|---|---|
| MOX | CEF | MOX | CEF | |
| Beer’s law range (μg /mL ) |
1-16 |
1-16 |
1-15 |
1-15 |
| Wavelength (nm) |
287.0 |
317.9 |
359.3 |
269.6 |
| Correlation Coefficent(r2) |
0.9992 |
0.9992 |
0.9991 |
0.9993 |
| Slope |
0.00362 |
0.00114 |
0.1807 |
0.0109 |
| Intercept |
5.08x10-4 |
−4.32x10-5 |
−0.0196 |
−0.0016 |
| LOD |
0.22 |
0.32 |
0.24 |
0.14 |
| LOQ |
0.75 |
0.95 |
0.70 |
0.43 |
| Intraday precision (%RSD) |
0.87 |
1.35 |
0.92 |
0.80 |
| Interday precision(%RSD) | 1.56 | 1.79 | 1.38 | 1.18 |
* First derivative Spectrophotometric method. **First derivative of the ratio spectra.
MOX-Moxifloxacin HCl, CEF- Cefixime trihydrate. %RSD : Percent Relative Standard Deviation.