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Radiochemical assay of UDP-Glc DH from soybean nodules. Samples were taken at the times indicated from a reaction mixture that contained 5 nmol of UDP-[U14C]Glc (6.8 nCi), subjected to paper chromatography, and analyzed for radioactivity as described in Methods. The amounts of UDP-GlcUA that were formed after 2, 10, and 20 min were 0.06, 0.14, and 0.19 nmol, respectively. The data are from one of two duplicate experiments.
