Table 1.

Primers and restriction enzymes for PCR-RFLP analysis

SNP	Primers	Annealing Temperature (°C)	PCR Product Size (bp)	Restriction Enzyme	Fragment Size After Enzymatic Cleavage (bp)
rs708567	Forward Primer: 5’-AGTAGGGTAGGCCTGGAAGG-3’	57	210	HinfI	GG: 210
	Reverse Primer: 5’-CACTGGGAAGAGCCTGAAGA-3’				AG: 161 + 49

*PCR-RFLP, polymerase chain reaction restriction fragment length polymorphism; SNP, single-nucleotide polymorphism.