Figure 6. Neutralizing antibodies elicited in the Env expressing LC16m8 Δ prime/SeV boost regimen.

(A) ID₅₀ of individual mouse serum against SF162 envelope-pseudotyped virus was determined using TZM-bl cells. Groups I, G and J consisted of 21, 17 and 14 mice, respectively. (B) Antibodies from randomly selected mice sera from group G, I, J and control were purified using protein A Sepharose. ID₅₀ of individual mouse serum against SF162 envelope-pseudotyped virus vs 50% inhibitory concentration (IC₅₀) (concentration of purified IgG that caused a 50% reduction in the RLU compared to virus control) of individual purified IgG was plotted. The regression line and R square are shown.