Table 1.
LC-MALDI-TOF-TOF results
| Workflow | No. of MS Spectraa | No. of total MS/MS Spectra | No. of MS/MS Spectra (mean of all LC-run) | Total identified Peptides | Non redundant identified peptidesb | Mean of proteins ± SD (n total protein)c | MS/MS efficiency (%)d | Peptides/ Proteins ± SD | Proteins identified by a single peptide (%) |
|---|---|---|---|---|---|---|---|---|---|
| Anionic |
125 |
7100 |
888 |
2700 |
1305 |
163 ± 87.6 (326) |
38% |
3.8 ± 4.3 |
15 (4,6%) |
| Cationic |
125 |
5832 |
729 |
1432 |
718 |
94 ± 39.6 (188) |
26% |
3.9 ± 4.4 |
11 (1,5%) |
| Lipophilic | 125 | 6554 | 819 | 1480 | 691 | 99 ± 5.6 (198) | 23% | 4.0 ± 4.2 | 9 (1,3%) |
a) number of total MS spectra/workflow; b) Peptide counts are based on non- redundant identifications in technical replicates and/or in redundant acquisitions of the same sample; c) Mean ± SD of identified proteins in four and three technical replicates for SPE-LC-MS/MS analysis and in brackets number of total identified proteins; d) Efficiency is determined dividing the number of identified peptides per MS/MS spectra.