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A. Negative-stain EM of liposomes prepared via sonication/ freeze-thaw (SFT), rapid solvent exchange (RSE), and freeze-thaw (FT) methods. B. Membrane deformation activities of various pacsin-1 constructs (5–10 µM) and CIP4F-BAR (10 µM) in Folch liposomes prepared following three different methods. Incubations and imaging were carried out as described previously.
