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. 2012 Dec 7;7(12):e51534. doi: 10.1371/journal.pone.0051534

Figure 4. Quantitative analysis of the nucleic acid content of viral particles released from MuLV producer cells.

Figure 4

(A) Quantitation of viral gRNA incorporated in wt or mutant viruses by RT-QPCR. Mock controls were subtracted from assays. Error bars indicate SD from at least four independent experiments. (B) Viral DNA levels were determined by qPCR in the wt and mutant virions. DNA was extracted from same virion samples as those used before for gRNA quantitation. Error bars indicate SD from at least seven independent experiments. (C) There is no correlation between gRNA and viral DNA levels among the MuLV mutants. For comparative purpose, data obtained with HIV-1 virions deleted of the second ZF (ΔZF2) are given (left part) [26], [38]. To facilitate the comparison, levels of viral gRNA and ss-cDNA were normalized to those measured in wt virions.