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Supernatant were collected from cells cotransfected with MuLV and wt or ΔZF2 HIV-1 molecular clones (MuLV:HIV ratio of 1∶3). Released virions were pelleted and proteins analyzed by Western blotting (A). The same blot was used to probe the MuLV and HIV-1 CA proteins. The intravirion levels of MuLV and HIV-1 DNA were determined and calculated as in Fig 4C (B).
