Figure 2. Co-cultured spheroids had higher viability and aggregated more rapidly than mono-cultured spheroids.

Spheroidal formation and viability of cells in mono-cultured spheroids cultured for (A) 3 days, (B) 7 days and in co-cultured spheroids cultured for (C) 3 days and (D) 7 days in concave microwells. Scale bars, 500 µm, (E) Diameter analysis of mono-cultured spheroids and co-cultured spheroids in 500 µm concave microwells. Data represent the means ± standard deviations of 10 independent experiments (*p<0.0001, two-tailed test).