Table 1. Protein identifications using Mascot and PEAKS.
| Scaffold | Peaks | SP | H | ||||||
| ID | Description | USC | CO | −10lgP | SC | USC | CO | ||
| Toxins | |||||||||
| 160380600 | Toxin CfTX-1 | 26 | 41 | 359.39 | 42 | 21 | 54 | + | + |
| 160380601 | Toxin CfTX-2 | 16 | 43 | 327.90 | 31 | 23 | 63 | + | + |
| 18146993 | Toxin CqTX-A | 2 | 23 | 224.45 | 16 | 1 | 27 | + | + |
| Nematogalectins | |||||||||
| 219233028 | Nematogalectin-related | - | - | 148.12 | 3 | 1 | 14 | + | + |
| 156401683 | Nematogalectin-related | 5 | 17 | 124.05 | 4 | 4 | 12 | + | + |
| 308210726 | Nematogalectin | - | - | 93.89 | 2 | 1 | 14 | + | + |
| Structural | |||||||||
| 159254 | Actin | 12 | 38 | 190.23 | 7 | 1 | 28 | − | + |
| 26522784 | Actin | - | - | 194.30 | 9 | 1 | 23 | − | + |
| 282471169 | Actin | - | - | 137.66 | 3 | 1 | 16 | − | + |
| 162122963 | Actin | - | - | 108.89 | 3 | 1 | 22 | − | + |
| 294372631 | Non-muscle actin | - | - | 158.11 | 4 | 1 | 25 | − | + |
| 156223974 | Alpha tubulin | 16 | 44 | 77.48 | 4 | 1 | 12 | − | + |
| 221129327 | Beta tubulin | 16 | 39 | 206.15 | 15 | 3 | 45 | − | + |
| 221129323 | Beta tubulin | - | - | 129.01 | 5 | 1 | 11 | − | + |
| 156369515 | Tubulin beta-2C chain | - | - | 89.33 | 3 | 3 | 14 | − | + |
| 225547767 | Clathrin heavy chain | 2 | 7 | - | - | - | - | − | − |
| 221130531 | Clathrin heavy chain 1 | 2 | 2 | 124.69 | 3 | 3 | 2 | − | − |
| 221107857 | Histone H2B-1 | - | - | 69.50 | 2 | 1 | 23 | − | + |
| 156219637 | Histone H2B | - | - | 126.82 | 6 | 1 | 30 | − | + |
| 156219675 | Histone H3.2-like | - | - | 81.04 | 3 | 2 | 11 | − | + |
| 156198376 | Histone H2A | 2 | 12 | 137.98 | 7 | 3 | 36 | − | + |
| 163678147 | Histone 2 | - | - | 62.91 | 2 | 1 | 15 | − | + |
| Heat shock proteins | |||||||||
| 221132017 | Heat shock protein | 3 | 4 | 88.74 | 2 | 1 | 10 | + | − |
| 262478028 | 90 heat-shock protein | 2 | 12 | - | - | - | - | − | + |
| Oxido-reductive | |||||||||
| 100913623 | Cytochrome c oxidase subunit II | 2 | 11 | - | - | - | - | − | − |
| 156364605 | ATP synthase beta subunit | 2 | 10 | - | - | - | - | − | + |
| 156213313 | ATP synthase | 5 | 18 | - | - | - | - | − | + |
| 162106843 | ATP synthase subunit alpha | - | - | 103.04 | 3 | 3 | 13 | − | + |
| 82862750 | V-type proton ATPase | - | - | 52.02 | 2 | 1 | 20 | − | + |
| 221111162 | Thioredoxin peroxidase | 2 | 10 | - | - | - | - | − | + |
| Dickkopf | |||||||||
| 221115495 | Dickkopf protein 3 | 4 | 38 | 216.04 | 11 | 9 | 38 | + | + |
| 37498690 | Dickkopf-3 related protein | 5 | 27 | 237.58 | 12 | 11 | 53 | + | + |
| Proteases | |||||||||
| 221116853 | Neprilysin | 3 | 4 | 116.69 | 2 | 1 | 3 | + | + |
| 221128287 | Neprilysin | - | - | 102.93 | 2 | 1 | 3 | + | + |
| 221132105 | Endothelin-converting enzyme 2 | - | - | 85.94 | 2 | 2 | 3 | + | + |
| 156386196 | Endothelin-converting enzyme 2-like | - | - | 56.17 | 2 | 2 | 3 | + | + |
| Miscellaneous | |||||||||
| 221118656 | DNA-directed RNA polymerase II | 2 | 1 | 189.76 | 9 | 9 | 3 | + | + |
| 156223696 | Ras-related protein Rab-35 | 2 | 12 | - | - | - | - | − | − |
| 239736186 | Elongation factor-1 alpha | 8 | 22 | 64.17 | 2 | 2 | 5 | − | − |
| 221129496 | DNA-directed RNA polymerase, omega | - | - | 120.92 | 2 | 2 | 1 | − | + |
| 221119922 | Gamma-glutamyltranspeptidase 1 | - | - | 66.25 | 2 | 2 | 2 | + | + |
| 221120305 | Gamma-glutamyl hydrolase | - | - | 67.12 | 2 | 2 | 3 | − | + |
| Unknown | |||||||||
| 221123368 | Hypothetical protein [Hydra magnipapillata] | 2 | 2 | 135.12 | 4 | 4 | 3 | + | − |
| 221113955 | Hypothetical protein [Hydra magnipapillata] | 2 | 14 | 124.91 | 2 | 2 | 14 | + | − |
| 221116631 | Predicted protein [Hydra magnipapillata] | 2 | 1 | 107.93 | 2 | 2 | 1 | − | − |
Protein identifications using Mascot and PEAKS. Abbreviations used: ID — identification number corresponding to custom database supplied as S5; USC — number of unique and significant spectra contributing to the identification; SC — total number of significant spectra contributing to the identification; CO — percent cover; and −10lgP — the PEAKS probability based score. A ‘+’ in the SP column denotes the presence of a signal sequence and a ‘+’ in the ‘H’ column denotes the identification of a similar protein in the H. magnipapillata venom proteome.