Figure 1. Expression of SRPK1 protein in ovarian cancer cell lines sensitive or resistant to cDDP.

(A) Quantitative real time reverse-transcription polymerase chain reaction (RT-PCR) analysis of SRPK1 RNA level in 6 OVCa cells and IOSE cells. GAPDH was used for loading controls and normalization. Fifty percent growth inhibition concentration (IC₅₀, µM) of cDDP for the OVCa cells was indicated lower panel). (B) Western blot analysis of SRPK1 protein in 6 OVCa cells and IOSE cells. The lower band in the blot probed with anti-SRPK1 antibody is probably proteolytic fragment of SRPK1. The blots were re-probed with actin which was used as a loading control and for normalization. Graph represents relative expression determined by densitometric measurement of the bands and is expressed relative to actin. Bar-graph shown is the average of 4 experiments. Overexpression of SRPK1 in OVCa was defined as greater than 2 fold over the mean of the IOSE samples and is marked with asterisks.