Skip to main content
. 2012 Dec 7;7(12):e50442. doi: 10.1371/journal.pone.0050442

Figure 6. HCN2 immunointensity in L4 DRGs 1 and 4 days after inflammation.

Figure 6

A to C: Representative images of ABC HCN2 staining in normal L4 DRG (A) and 1 day (B) or 4 days (C) after CFA-induced cutaneous inflammation. Note the elevation in the overall staining of small neurons by 1 day and the weaker HCN2 ring and cytoplasmic staining in medium and large neurons by 4 days. D and E: Neurons subdivided into small, medium and large (cross sectional areas with nuclear profiles of <400 µm2, 400–800 µm2, >800 µm2 respectively). Cytoplasmic staining: pixel densities over entire cytoplasm; edge staining: pixel densities under line drawn over cell perimeter. Comparison of cytoplasmic (D) and edge (E) staining in sections of normal (N) L5 DRGs with ipsilateral L5 DRGs 1 day (1) and 4 days (4) after CFA-induced cutaneous inflammation. Kruskall-Wallis of N, 1 and 4 groups within each size range with Dunn’s post-hoc test between each possible pair. For significances see Fig. 5 legend.