Fig. 15.

Plasma free heme concentrations following induction of AKI. During the initiation phases of I/R, glycerol, or cisplatin induced AKI (4, 4, and 24 h, respectively) an ∼3-fold increase in plasma free heme was observed, compared with values observed in control mouse plasma. Upon reaching the so called maintenance phase of I/R or cisplatin AKI (24 h and 72 h respectively), the plasma free heme levels fell, reaching values that were approximately two-thirds lower than were observed in normal mouse plasma. These falling free heme levels corresponded to rising plasma Hpx levels. In contrast, free heme concentrations did not fall between 4 by 24 h postglycerol injection. This was likely due to a relative failure of plasma Hpx upregulation during this period (see Fig. 3). Finally, LPS injection led to a 6-fold increase in free heme by 24 h postinjection.