Fig. 9.

Hpx mRNA stability, as assessed in isolated proximal tubules. Isolated tubules were harvested from control and LPS treated mice and incubated for 90 min with actinomycin D to assess mRNA degradation rates. LPS induced marked Hpx mRNA stabilization, as indicated by ∼80% retention of control mRNA levels (vs. 35% retention in control tubules). For comparison, TNF-α mRNA was not stabilized by LPS pretreatment (right). This indicates that Hpx mRNA stabilization was not a completely nonspecific event.