Table 1.

Primers used to detect mouse and human Hpx mRNA

mRNA	Primer Sequences	Product Size
Human primers for RT-PCR
Hpx	5′-TCTGCACTGACGTCTGACAACCAT-3′	307 bp
	5′-AGATAAAGGCCGCATCCACAGAGT-3′
GAPDH	5′-AAGGTCGGAGTCAACGGATTTGGT-3′	534 bp
	5′-AGTGATGGCATGGACTGTGGTCAT-3′
Mouse primers for RT-PCR
Hpx	5′-ACACTGCTTGGATACCTGGAGCTT-3′	854 bp
	5′-TGCCTCCCTTTGTCAGGAAGACAT-3′
GAPDH	5′-CTGCCATTTGCAGTGGCAAAGTGG-3′	437 bp
	5′-TTGTCATGGATGACCTTGGCCAGG-3′

Primer pairs that were employed to detect mouse hemopexin (Hpx) mRNA in renal cortical samples and human Hpx mRNA in RNA extracts from HEK 293 and HK-2 cells are presented. mRNAs were assessed by competitive RT-PCR, with the results being factored by simultaneously obtained GAPDH product.