Fig. 1.

Leucine supplementation prevents high-fat diet (HFD)-induced obesity. Mice were fed normal diet (ND) or HFD, supplemented with/without leucine (Leu, 1.5 g/100 ml in drinking water) for 8 wk. A: leucine intake was calculated from the amount of drinking water in individually caged mice; n = 30 in each group. *P < 0.05 vs. ND or HFD. B: serum leucine concentrations were analyzed as described in materials and methods; n = 10 in each group. *P < 0.05 vs. ND or HFD. C: daily caloric intake was calculated from the amount of ingested food in individually caged mice; n = 30 in each group. *P < 0.05 vs. ND or leucine. D: body weights were monitored at weeks indicated; n = 30 in each group. *P < 0.05. E: subcutaneous and visceral fat was measured after 8 wk of treatment; n = 10 in each group. *P < 0.05 vs. ND or Leu; †P < 0.05 HFD-Leu vs. HFD.