Fig. 5.

Effects of HFD and leucine on SIRT1 signaling and expression of FAO genes in skeletal muscle. A: homogenates of gastrocnemius muscle were subjected to WB analysis using an anti-SIRT1 antibody (n = 5). *P < 0.05 vs. ND or HFD. B: RNA was extracted from gastrocnemius muscle, and quantitative real-time PCR was performed to determine SIRT1 mRNA levels. C: homogenates of gastrocnemius muscle were subjected to WB analysis to detect NAMPT expression (n = 5). *P < 0.05 vs. ND; †P < 0.05 vs. HFD. D: PGC1α was immunoprecipitated and Western blotted with an acetylated lysine antibody. E: muscle homogenates were immunoprecipitated by anti-FoxO1 antibody, and acetylated lysine was detected by WB analysis. F: relative mRNA expression levels of genes related to FAO (n = 5 in each group). *P < 0.05 vs. HFD.