Fig. 8.

Leucine supplementation improves insulin sensitivity in mice subjected to HFD. A: fasting blood glucose levels were measured in tail vein blood sample using a glucometer (n = 8 in each group). *P < 0.05 vs. ND; †P < 0.05 vs. HFD. B: an intraperitoneal glucose tolerance test was performed in mice after an overnight fast (n = 5 per group). *P < 0.05. C: area under the curve (AUC) was calculated for each dietary condition (n = 5 per group). *P < 0.05 vs. ND; †P < 0.05 vs. HFD. D and E: hyperinsulinemic-euglycemic clamps were performed over a 120-min period as described in materials and methods. Insulin sensitivity was evaluated through the average glucose infusion rate at equilibrium in a hyperinsulinemic-euglycemic clamp (18 mU insulin·min⁻¹·kg⁻¹; n = 5 in each group). *P < 0.05 vs. ND; †P < 0.05 vs. HFD.