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Nicotinate receptor GPR109A as potential mediator of butyrate effects on MCT1 function. GPR109A protein expression in IEC-6 and C2BBe1 cells (75 μg protein in cell lysate was loaded, probed with anti-human GPR109A antibody) (A); time-course of 100 μM nicotinate effects on MCT1 function (B); and time-course of nicotinate effects on intracellular cAMP (n = 3, *P < 0.05) (C).
