Relative expression of RF transporter-1 (RFVT-1), RFVT-2, and RFVT-3 in mouse-derived pancreatic β-TC-6 cells (A), freshly isolated primary mouse pancreatic islets (B), and freshly isolated human pancreatic islets (C). Total RNA were isolated from cultured β-TC-6 cells (A), freshly isolated primary mouse (B), and human pancreatic islets (C) and were subjected to quantitative real-time PCR analysis using RFVT-1, -2, and -3 gene-specific primers (Table 1). Levels are expressed relative to β-actin. Values are means ± SE of at least 3 independent experiments. *P < 0.01.