Table 3.
Comparison of mRNA and protein expression of amino acid transporters in response to resistance exercise coupled with essential amino acid ingestion or resistance exercise alone in skeletal muscle of young men.
| Study Group | Basal | 3h Post RE | 6h Post RE |
|---|---|---|---|
| LAT1/SLC7A5 mRNA, Fold Change | |||
| RE+EAA | 1.11 ± 0.23 | 3.95 ± 0.75* | 3.74 ± 0.86*# |
| RE | 1.01 ± 0.16 | 2.64 ± 0.49* | 1.84 ± 0.35 |
| SNAT2/SLC38A2 mRNA, Fold Change | |||
| RE+EAA | 1.04 ± 0.13 | 1.96 ± 0.39* | 1.21 ± 0.37 |
| RE | 1.23 ± 0.35 | 1.54 ± 0.18 | 1.19 ± 0.32 |
| PAT1/SLC36A1 mRNA, Fold Change | |||
| RE+EAA | 1.13 ± 0.21 | 1.66 ± 0.49 | 2.00 ± 0.72‡ |
| RE | 1.02 ± 0.22 | 1.01 ± 0.20 | 2.22 ± 0.48 |
| CAT1/SLC7A1 mRNA, Fold Change | |||
| RE+EAA | 1.12 ± 0.19 | 1.61 ± 0.29 | 3.88 ± 0.93* |
| RE | 1.27 ± 0.19 | 1.85 ± 0.20 | 4.84 ± 1.09* |
| CD98/SLC3A2 mRNA, Fold Change | |||
| RE+EAA | 1.08 ± 0.20 | 1.48 ± 0.42 | 1.68 ± 0.42‡ |
| RE | 1.20 ± 0.24 | 0.91 ± 0.12 | 1.89 ± 0.37‡ |
| LAT1 Protein, Fold Change | |||
| RE+EAA | 1.00 | 0.84 ± 0.09* | 0.96 ± 0.12 |
| RE | 1.00 | 1.12 ± 0.07# | 1.25 ± 0.07*# |
| SNAT2 Protein, Fold Change | |||
| RE+EAA | 1.00 | 1.40 ± 0.11*# | 1.63 ± 0.22*# |
| RE | 1.00 | 1.01 ± 0.01 | 1.01 ± 0.01 |
Data are mean±SE. RE+EAA = resistance exercise followed by ingestion of 20 g of essential amino acids 1h following exercise; RE = resistance exercise without EAA ingestion. RE+EAA, n = 6; RE, n = 8.
*
Significant change from basal (p<0.05);
#
Significant group difference (p<0.05);
‡
Significant time effect vs. basal (p<0.05).