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. 2012 Aug 29;303(9):R941–R949. doi: 10.1152/ajpregu.00336.2012

Fig. 2.

Fig. 2.

Compromise of GPR107, but not GPR56, in KATOIII cells results in a loss of neuronostatin-induced expression of c-Fos. A: transfection of KATOIII cells with siRNA directed against GPR56 led to a concentration-related decrease in GPR56 expression, as determined by RT-PCR. B: treatment of KATOIII cells transfected with GPR56 siRNA resulted in a significant increase in c-Fos expression similar to that observed in vehicle-transfected cells. C: transfection of KATOIII cells with siRNA directed against GPR146 led to a concentration-related decrease in GPR146 mRNA levels. D: knockdown of endogenous GPR146 in KATOIII cells did not affect neuronostatin-induced c-Fos expression. E: Transfection of KATOIII cells with siRNA directed against GPR107 led to a concentration-related decrease in GPR107 RNA levels. F: transfection with siRNA directed against GPR107 abolished neuronostatin-induced c-Fos expression in KATOIII cells. Data are presented as percent of control (vehicle-transfected, vehicle-treated cells). *P < 0.05, **P < 0.01, ***P < 0.001 vs. control. #P < 0.05 vs. GPR107 siRNA-transfected, neuronostatin-treated cells.