Table 1.
Human primer sequences
| Target Name | RefSeq ID | Forward Primer | Reverse Primer | Tm (F, R) | Amplicon Size | Location on Sequence (F, R) |
|---|---|---|---|---|---|---|
| LPAR5 | NM_020400.5 | AGGGCAATGCTTCCTCTTCACTCT | AGGCTGTACAGACATGGTCCCAAA | 58, 58 | 332 | 19, 350 |
| OXGR1 | NM_080818.3 | CCACGAACGGGTAATCCTGATGAA | GTCTAGTGGCTCATTCATGGTTGTC | 57, 56 | 153 | 110, 262 |
| SUCNR1 | NM_033050.4 | TGGCAGAGTTCCTGTCAAGGGAT | AGGACTCCCACAACGAACTCAATC | 57, 57 | 199 | 23, 221 |
| GPR15 | NM_005290.1 | AGGGAGCTCACGCTGATTGATGAT | TATTGAAGGGCAGCCAGGAGACAA | 58, 58 | 262 | 514, 775 |
| GPR17 | NM_001161415.1 | TCAGGATGTCCAAACGGAGTTGGT | CAGAGCCAGGGTATTGCCAACTAA | 58, 57 | 248 | 607, 854 |
| GPR18 | NM_005292.3 | ACAGGAGGTGCTGCTTTCTGTGAA | ACGTCCTGCCGTGAAGGAGATTAT | 58, 58 | 891 | 269, 1159 |
| GPR19 | NM_006143.2 | TCTGCCAAGCCAATACCTGATGGA | CATCAAAGATCCACGATGCCGCAA | 58, 58 | 473 | 457, 929 |
| GPR22 | NM_005295.2 | ACCAGGGAATAGGTCTACATTACTGA | GTCATCAATGTCATCTCGCACTGT | 55, 56 | 898 | 524, 1421 |
| GPR26 | NM_153442.3 | AACTGTGGCACATGCTCTTTCTGC | TCACCCTGGAGTGAAGTCAAGCAA | 60, 60 | 535 | 3309, 3843 |
| GPR31 | NM_005299.2 | TAAGGTCAACCTGCTGTCTCCTCA | CATTGCAGAACACGATGAGGCCAA | 57, 58 | 230 | 363, 592 |
| GPR32 | NM_001506.1 | TCCTGACACGTGATCGCTCTTGTT | AATGGGCAGAGACAGTGAGAGCAT | 58, 58 | 239 | 53, 291 |
| GPR55 | NM_005683.3 | TCCTTAAGAACAGGTGGCCCGATT | TGTTTCTCACCAGGAACTGCAGGA | 58, 58 | 621 | 336, 956 |
| GPR56 | NM_005682 | CCAGCACCAGCTACAGCCGAAGAA | TGGAGCAGAGGTAGGCGGCAAT | 61, 60 | 287 | 1291, 1577 |
| GPR63 | NM_030784.2 | ATGGTCTTCTCGGCAGTGTTGACT | TAAGCCTGGTAGCCTGGATTGGTT | 58, 58 | 725 | 361, 1085 |
| GPR82 | NM_080817.4 | GGTGTCATGCAAGAATCTATTCACAC | TGCAATCTGAGAGATCATGGCTCC | 55, 57 | 842 | 5, 846 |
| GPR83 | NM_016540.3 | ACCTTCTCCGACTGGCAGAACTTT | TGGCAGATAGCATGTGGGAGTGAA | 58, 58 | 482 | 314, 795 |
| GPR85 | NM_001146265.1 | TCAGGCTTTGTGGTGTCTGTGGTA | TCAGAAAGGCTGTTAGAGGCGAGA | 58, 57 | 400 | 294, 694 |
| GPR107 (PCR) | NM_001136557.1 | AGTGGAAGTGGCTCTACCAGCTC | TCACTGTCGGTGGTGCCAATAGTT | 58, 58 | 350 | 1792, 2141 |
| GPR107 (RT-PCR) | NM_001136557.1 | TGGCTTAGAAGGGTCAGGCAGAAA | ATGGGACAGGGAGAACAACCATGT | 58, 58 | 112 | 3485, 3596 |
| GPR146 | NM_138445.2 | ACTTTGTCAACATGGCAGTGGCAG | TGGACACATGGCTGCAGATGTAGA | 58, 58 | 324 | 205, 528 |
| GPR160 | NM_014373.2 | TTGCGCAAATGTCTCCGAGCTTAC | AGCATTCTGTGCCTTCAGGCTTTG | 58, 58 | 716 | 382, 1097 |
| GPR176 | NM_007223.1 | TCCTCAGCTTCCCTGCTATTGCTT | AGGGCACGCTACACAAGATGAAGA | 58, 58 | 450 | 1261, 1710 |
| GPR183 (EB12) | NM_004951.4 | ACTCACAGCTACTACACAGAGACC | CCAGTCAAAGCCCATTGCATAGTAGG | 55, 58 | 351 | 115, 465 |
| GAPDH | NM_002046.3 | TCACTGCCACTCAGAAGACTGT | CGTTCAGCTCTAGGATGACCTT | 58, 56 | 135 | 644, 778 |
| cFos | NM_005252.3 | GGACTCAAGTCCTTACCTCTTCC | CCTGGCTCAACATGCTACTAACT | 56, 57 | 106 | 1524, 1629 |