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. 2012 Sep 28;26(12):1967–1979. doi: 10.1210/me.2012-1165

Fig. 4.

Fig. 4.

Vav3 and AR3 (AR-V7) are critical for anchorage-dependent and anchorage-independent growth. A, 22Rv1 cells stably expressing plk0.1-shGFP, -shVav3, -shAR3, or -shAR were plated at 20,000 cells per well in 24-well dishes in 10% FBS or 5% CSS. Cells were harvested and counted at three distinct time points. Data represent three independent experiments performed in triplicate and are plotted as mean doubling time (hours) ± sem as determined by the following equation: doubling time = ln(2)/growth rate. Cell lysates were immunoblotted with Vav3 or N-terminal AR and actin antibodies. Significance was determined using a Student's t test of comparison with shGFP controls. *, P < 0.05; **, P < 0.01. B, The growth in soft agar of 22Rv1 cells stably expressing the indicated shRNA vectors was assessed as described in Materials and Methods. Average colony number per plate ± sem is plotted. Data represent two independent experiments performed in triplicate.