Table 1.
Knockdown of Vav3 or AR3 (AR-V7) in CRPC cells results in G0/G1 accumulation
| Phase |
|||
|---|---|---|---|
| G0/G1 | S | G2 | |
| shGFP | 68 (1.1) | 13 (1.1) | 19 (1.0) |
| shVav3 | 79b (0.6) | 7a (0.8) | 13a (1.4) |
| shAR3 | 81b (1.7) | 6b (0.2) | 13a (1.4) |
| shAR | 70 (1.9) | 12 (0.8) | 18 (1.5) |
The 22Rv1 cells stably expressing plk0.1-shGFP, -shVav3, -shAR3, or -shAR were plated at one million cells per 100-mm dish. Cells were grown in androgen-depleted media for 24 h, and then media were changed to 10% FBS for 48 h. Cells were fixed in 70% ethanol, and DNA content was determined with propidium iodide and flow cytometry. Data (sem) represent three independent experiments performed in triplicate. Significance was determined using a Student's t test of comparison with the shGFP-expressing controls.
a
P < 0.05.
b
P < 0.01.