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. 2012 Oct 1;26(12):1991–2003. doi: 10.1210/me.2012.1227

Fig. 1.

Fig. 1.

Stable expression of ERβ1 and ERβ2. A and B, qPCR analysis of ERβ1 and ERβ2 mRNA in stably transfected PC3 cells. Control +Dox (Doxycycline) (bar 1), control −Dox (bar 2), ERβ1/β2 +Dox (bar 3), and ERβ1/β2 −Dox (bar 4). C, The protein expression of ERβ1 and ERβ2 was verified by Western blotting using the ERβ antibody 14C8 (Abcam). Lane 1 represents the control cells, lane 2 ERβ1-expressing cells, and lane 3 ERβ2-expressing cells. The FLAG-ERβ2 band is slightly lower than the ERβ1 band. β-Actin was used as a loading control. D and E, qPCR analysis of ERβ1 and ERβ2 mRNA in stably transfected 22Rv1 cells. All the experiments were performed n = 3.