Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1981 Jan;31(1):223–227. doi: 10.1128/iai.31.1.223-227.1981

Outer membrane proteins induced under conditions of iron limitation in the marine fish pathogen Vibrio anguillarum 775.

J H Crosa, L L Hodges
PMCID: PMC351773  PMID: 7194320

Abstract

Cells of the marine fish pathogen Vibrio anguillarum 775 harboring a plasmid associated with virulence can grow unaffected in the presence of iron-binding compounds such as transferrin. In contrast, the growth of isogenic plasmidless derivatives is inhibited by the presence of iron chelators. Radioactive from (55Fe3+) uptake experiments indicate that this plasmid-linked ability of V. anguillarum cells to grow under conditions of iron limitation is indeed due to a more rapid and efficient iron uptake mediated by the virulence plasmid. In addition, V. anguillarum cells growing under iron limitation show at least two novel outer membrane proteins. One of them, a 86,000-dalton protein we called OM2, is inducible only in those cells in which the virulence plasmid is present.

Full text

PDF
224

Images in this article

225Image
on p.225

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bennett R. L., Rothfield L. I. Genetic and physiological regulation of intrinsic proteins of the outer membrane of Salmonella typhimurium. J Bacteriol. 1976 Jul;127(1):498–504. doi: 10.1128/jb.127.1.498-504.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cisar J. O., Fryer J. L. An epizootic of vibriosis in chinook salmon. Wildl Dis. 1969 Apr;5(2):73–76. doi: 10.7589/0090-3558-5.2.73. [DOI] [PubMed] [Google Scholar]
  3. Cox C. D. Iron uptake with ferripyochelin and ferric citrate by Pseudomonas aeruginosa. J Bacteriol. 1980 May;142(2):581–587. doi: 10.1128/jb.142.2.581-587.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Crosa J. H. A plasmid associated with virulence in the marine fish pathogen Vibrio anguillarum specifies an iron-sequestering system. Nature. 1980 Apr 10;284(5756):566–568. doi: 10.1038/284566a0. [DOI] [PubMed] [Google Scholar]
  5. Crosa J. H., Hodges L. L., Schiewe M. H. Curing of a plasmid is correlated with an attenuation of virulence in the marine fish pathogen Vibrio anguillarum. Infect Immun. 1980 Mar;27(3):897–902. doi: 10.1128/iai.27.3.897-902.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Crosa J. H., Olarte J., Mata L. J., Luttropp L. K., Peñaranda M. E. Characterization of an R-plasmid associated with ampicillin resistance in Shigella dysenteriae type 1 isolated from epidemics. Antimicrob Agents Chemother. 1977 Mar;11(3):553–558. doi: 10.1128/aac.11.3.553. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Crosa J. H., Schiewe M. H., Falkow S. Evidence for plasmid contribution to the virulence of fish pathogen Vibrio anguillarum. Infect Immun. 1977 Nov;18(2):509–513. doi: 10.1128/iai.18.2.509-513.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Ernst J. F., Bennett R. L., Rothfield L. I. Constitutive expression of the iron-enterochelin and ferrichrome uptake systems in a mutant strain of Salmonella typhimurium. J Bacteriol. 1978 Sep;135(3):928–934. doi: 10.1128/jb.135.3.928-934.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hancock R. E., Hantke K., Braun V. Iron transport of Escherichia coli K-12: involvement of the colicin B receptor and of a citrate-inducible protein. J Bacteriol. 1976 Sep;127(3):1370–1375. doi: 10.1128/jb.127.3.1370-1375.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Ichihara S., Mizushima S. Identification of an outer membrane protein responsible for the binding of the Fe-enterochelin complex to Escherichia coli cells. J Biochem. 1978 Jan;83(1):137–140. doi: 10.1093/oxfordjournals.jbchem.a131884. [DOI] [PubMed] [Google Scholar]
  11. Ichihara S., Mizushima S. Involvement of outer membrane proteins in enterochelin-mediated iron uptake in Escherichia coli. J Biochem. 1977 Mar;81(3):749–756. doi: 10.1093/oxfordjournals.jbchem.a131513. [DOI] [PubMed] [Google Scholar]
  12. Langman L., Young I. G., Frost G. E., Rosenberg H., Gibson F. Enterochelin system of iron transport in Escherichia coli: mutations affecting ferric-enterochelin esterase. J Bacteriol. 1972 Dec;112(3):1142–1149. doi: 10.1128/jb.112.3.1142-1149.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. McIntosh M. A., Earhart C. F. Coordinate regulation by iron of the synthesis of phenolate compounds and three outer membrane proteins in Escherichia coli. J Bacteriol. 1977 Jul;131(1):331–339. doi: 10.1128/jb.131.1.331-339.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Schiewe M. H., Crosa J. H., Ordal E. J. Deoxyribonucleic acid relationships among marine vibrios pathogenic to fish. Can J Microbiol. 1977 Aug;23(8):954–958. doi: 10.1139/m77-142. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES