Figure 4.
Over-expression of TBX3 increases stem cell proliferation possibly by repressing the expression of NFκBIB and p14ARF. (A) Phase images of control (left) and TBX3 over-expressing (right) human ESC colonies cultured on MEFs. Examples of DAPI images used to calculate mitotic index. Mitotic cells are shown in insets. (B) TBX3 over-expressing ESCs have a significantly higher mitotic index than control ESCs. *, p<0.05. (C) TBX3 over-expression increases the percentage of BrdU positive ESCs cells. *, p<0.05 (D) TBX3 over-expression in human ESCs results in a significant decrease in the expression of cell cycle regulators, NFκBIB and p14ARF. *, p<0.05.