TBX3 knockdown inhibits neuroepithelial, neuroectoderm and eye field marker expression. Expression levels of pluripotency and differentiation related markers in TBX3 knockdown cell lines at day 8 of differentiation with 10% FBS were analyzed. (A) Quantitative RT-PCR was performed on the expression of TBX3, endoderm markers (AFP and GATA4), mesoderm markers (BRACHYURY and HAND1) and neuroepithelial markers (PAX6 and SOX1). Control cells= 1.0. *, p<0.05. (B) Quantitative RT-PCR was performed on the expression of TBX3, pluripotency markers (OCT4, NANOG, and SOX2), neuroepithelial markers (PAX6 and SOX1), forebrain markers (FOXG1 and LHX2) and eye field markers (PAX6, LHX2, RAX). *, p<0.05.