TABLE 1.
Oligonucleotides for PCR amplification and sequencing of the human SLC4A1/AE1 gene.
| Oligo | SEQUENCE | Direction | Location | Use | Oligo location (nt per NG_007498) |
Amplicon Length |
|---|---|---|---|---|---|---|
| hAE1.I1F | 5′-GAGACCCTGGGATGCCCTGTG-3′ | Forward | Intron 1 | PCR-1, Seq | 10151-10161 | 1490 bp |
| hAE1.I4R | 5′-CCCTTGCTCCTCTCTTCCCTG-3′ | Reverse | Intron 4 | PCR-1, Seq | 11640-11620 | |
| hAE1.I4F | 5′-AAGCCTCACAAGCACAAGCCTC-3′ | Forward | Intron 4 | PCR-2, Seq | 12224-12245 | 1837 bp |
| hAE1.I9R | 5′-AGTAACCCAGGCCAGGTTGGAG-3′ | Reverse | Intron 9 | PCR-2, Seq | 14060-14039 | |
| hAE1.I6F | 5′-TGTCTACCCTTGATGTCTACC-3′ | Forward | Intron 6 | Seq (PCR-2) | 13090-13110 | -- |
| hAE1.I9F | 5′-TGTGGACTCCTGGGTCCTTTCC-3′ | Forward | Intron 9 | PCR-3, Seq | 14447-14467 | 1,440 bp |
| hAE1.I13R | 5′-CTTATACACAACCTCCCGTGTG-3′ | Reverse | Inron 13 | PCR-3, Seq | 15886-15865 | |
| hAE1.I13F | 5′-GCTGGGTAGAGTTAGCTGGTGG-3′ | Forward | Intron 13 | PCR-4, Seq | 17208-17229 | 1511 bp |
| hAE1.I16R | 5′-TCTTGCCTGCCTGGGAGAATGC-3′ | Reverse | Intron 16 | PCR-4, Seq | 18718-18697 | |
| hAE1.I16F | 5′-GCTACAAGGACACCAAGTATGG-3′ | Forward | Intron 16 | PCR-5, Seq | 19675-19696 | 3057 bp |
| hAE1.3R1 | 5′-TGGAAGGTGGGGATGTGGAATG-3′ | Reverse | Exon 20(a) | PCR-5, Seq | 22731-22710 | |
| hAE1.I17F | 5′-GTGCCTGTGTTTTATTCCCAGC-3′ | Forward | Intron 17 | Seq (PCR-5) | 21449-21470 | -- |
(a)
3′-UTR of the hAE1 mRNA