Figure 1.

Scheme of experimental design of the study. Embryos cultured from zygote to morula stage were subjected to 24 hours of simulated shipment (Experiment 3). Embryos developed in vivo were subjected to either 24 hours (Experiment 1) or 48 hours (Experiment 2) of simulated shipment. In all cases, embryo development to blastocyst stage and total cell number was reordered. For 24 hour experiments, embryo transfer to surrogate mother foster was performed. Additionally, in the 48 hour experiment, gene expression analysis was carried out.