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. 2012 Nov 23;12:548. doi: 10.1186/1471-2407-12-548

Figure 2.

Figure 2

Examples from screening of mCRC samples using the c.34 G > T CADMA assay. A. Real-time amplification data. High background fluorescence can be observed for sample ID 69. B. The derivative of the raw melting data (melt curve analysis). Sample ID 56 carry the c.34 G > T mutation. Sample ID 2, 69 and 72 were negative for the c.34 G > T mutation. Sample ID 69 and 72 may carry another KRAS mutation as small deviations from the wild-type replicates can be observed. Sample ID 69, which gave high fluorescence during the PCR amplification, has deviating melt curves from 82 to 88°C. C. Normalized HRM difference graph. Of the mCRC samples shown only one (sample ID 56) deviates more from the wild-type replicates than the standard containing 1% mutant alleles.