Table I.
Antioxidant | Common Bean
|
Cowpea
|
||
---|---|---|---|---|
Periphery | Infected zone | Periphery | Infected zone | |
Ascorbate | 124 ± 9 a | 86 ± 11 b | 50 ± 4 a | 30 ± 4 b |
(Homo)glutathionea | 37.8 ± 1.0 a | 26.3 ± 2.0 b | 26.4 ± 2.0 a | 12.0 ± 1.2 b |
AP | 1.08 ± 0.03 a | 1.01 ± 0.05 a | 1.40 ± 0.07 a | 1.38 ± 0.02 a |
Dehydroascorbate reductase | 51.6 ± 4.9 a | 29.1 ± 2.7 b | 35.1 ± 2.9 a | 40.2 ± 1.7 a |
Monodehydroascorbate reductase | 384 ± 16 a | 244 ± 5 b | 340 ± 22 a | 217 ± 9 b |
(Homo)glutathione reductaseb | 41.0 ± 2.9 a | 52.1 ± 1.2 b | 44.3 ± 2.2 a | 41.1 ± 1.4 a |
Catalase | 125 ± 3 a | 163 ± 5 b | 82 ± 8 a | 150 ± 12 b |
Metabolite contents are expressed as nmol mg−1 protein. Activities are expressed as μmol substrate min−1 mg−1 protein (catalase and AP) or in nmol substrate or product min−1 mg−1 protein (others). Values are means ± se (n = 4–7). For each species, means denoted by the same letter do not differ significantly at P = 0.05 based on Duncan's multiple-range test.
Reduced plus oxidized (homo)glutathione.
Assayed using oxidized glutathione as a substrate.