Table 1.

List of primers used in this study.

Name	Nucleotide Sequence	Description
p1	5′ -ccGCATGCTACACCATTGTTAGTTGTATACAAGG	Forward primer of pbcrt 3′ coding sequence; SphI site underlined
p2	5′ -ttGCGGCCGCTTATGCCCTTGATGTTTCTATAGAAG	Reverse primer of pbcrt 3′ coding sequence; NotI site underlined
p3	5′-ccAAGCTTTTGATACAACATTTTTATTTCTTAAATGATTTTTG	Forward primer of pbcrt 3′UTR;HindIII site underlined
p4	5′ -ccGCATGCCTCTCTATACATAGGCAAATAAGG	Reverse primer of pbcrt 3′UTR; SphI site underlined
p5	5′-CATATGTGATAATTTACTTGCTTGC	Forward primer of pbcrt upstream coding sequence
p6	5′-CTGGTGCTTTGAGGGGTGAGC	Reverse primer from hdhfr selectable marker cassette
p7	5′-CAGGAAACAGCTATGAC	Reverse primer specific to the plasmid backbone
p8	5′-GTTGGTTCGCTAAACTGCATC	Forward primer for hdhfr qPCR
p9	5′-CTGTTTACCTTCTACTGAAGAGG	Reverse primer for hdhfr qPCR
p10	5′-TGCAGCAGATAATCAAACTC	Forward primer for hsp70 qPCR
p11	5′-ACTTCAATTTGTGGAACACC	Reverse primer for hsp70 qPCR
p12	5′-CCTTATCTCATTATTAGATGCTTCTAC	Forward primer for pbcrt RT-PCR
p13	5′-CCAATATTCTTGGTTTTCTTACAGC	Reverse primer for pbcrt RT-PCR
p14	5′-TATGGGTCCAAGATATTGTAGTAATAA	Forward primer for Plasmodium berghei ama1 RT-PCR
p15	5′-GAATTAGCTTTACCATAAATATCTGC	Reverse primer for P. berghei ama1 RT-PCR
QT	5′-CCAGTGAGCAGAGTGACGAGGACTCGAGCTCAAGC(T)17	Primer to generate cDNA for 3′RACE
QO	5′-CCAGTGAGCAGAGTGACG	Outer primer for 3′RACE
QI	5′-GAGGACTCGAGCTCAAGC	Inner primer for 3′RACE
SP6	5′-ATTTAGGTGACACTATAG	pGEM vector-specific primer to sequence 3′RACE products
M13F	5′-GTAAAACGACGGCCAGT	pGEM vector-specific primer to sequence 3′RACE products

amal, apical membrane antigen 1; hdhfr human dihydrofolate reductase selectable marker; hsp70, heat shock protein 70; Pbcrt, Plasmodium berghei chloroquine resistance transporter; qPCR, quantitative PCR; RACE, random amplification of cDNA ends; RT-PCR, reverse transcription PCR; UTR, untranslated region.