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. Author manuscript; available in PMC: 2013 Aug 31.
Published in final edited form as: Circ Res. 2012 Jul 18;111(6):685–696. doi: 10.1161/CIRCRESAHA.112.269811

Figure 6. H3 acetylation of the SM22α promoter was reduced and was G/C Repressor dependent.

Figure 6

A&B). SM22 and SM22 G/C mut LacZ mice were subjected to right common carotid injury and then harvested 3 days after vascular injury, tissues from ten mice were pooled, and then subjected to ChIP assay for AcH3 (A) or HDAC2 (B). In each ChIP IP, qPCR analysis was conducted on both the Endogenous and LacZ transgene as indicated in the Figure. * indicates significant binding as mentioned in Fig 2D. C&D) Rat aortic smooth muscle cells were plated at 1×10^4, allowed to grow to confluency and then switched to serum free media for three days. Following serum starvation, cells were treated with 20 ng/ml PDGF-BB (C) or 5 ug/ml of POVPC (D) for 12 hours and then subject to ChIP analysis. * indicates significant binding as mentioned in Fig 2D.