Table 3.
Immunostaining results of the three different molecular markers of cell cycle in cardiomyocytes (CM) and VEC (vascular endothelial cells). Data is expressed as stimulation index (SI), defined by the (% labeled nuclei +TNF) − (% labeled nuclei −TNF)] / (% labeled nuclei −TNF) for each strain. TNF-treated TNFR1KO cultures show the highest SI index as compared to WTC56B/L controls or TNFR2KO. Data are from three independent experiments.
WTC57BL/6 | TNFR1KO | TNFR2KO | TNFR1/TNFR2KO | |
---|---|---|---|---|
Ki-67 CM | 3.37±0.62 | 8.60±1.92 | 0.37±0.12 | 0.38±0.27 |
Ki-67 VEC | 2.87±1.11 | 8.03±1.77 | 0.81±0.49 | 0.70±0.36 |
pH3 CM | 3.22±0.52 | 7.78±2.10 | 0.80±0.45 | 0.18±0.24 |
pH3 VEC | 3.39±0.98 | 6.55±1.30 | 0.48±0.37 | 0.43±0.36 |
PCNA CM | 2.80±0.63 | 9.97±1.99 | 0.52±0.26 | 0.38±0.30 |
PCNA VEC | 3.85±1.21 | 7.01±1.87 | 0.43±0.28 | 0.71±0.49 |