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. Author manuscript; available in PMC: 2013 Dec 1.
Published in final edited form as: J Virol Methods. 2012 Sep 4;186(1-2):68–72. doi: 10.1016/j.jviromet.2012.08.019

Figure 1.

Figure 1

Visual representation of ddPCR results for HIV-1 DNA standards after flow enumeration. Positive droplets (upper cluster) and negative droplets (lower cluster) are shown for 6 serially diluted linear DNA standards (A1 through F1) and negative control (G1). The Y axis represents fluorescent intensity and the X axis is the total number of events for each standard dilution. The manually set threshold for droplet positivity is represented by the horizontal line.