Table 3. HIV-1 2-LTR Circle and Human Mitochondria Copy Number Detected by ddPCR and RT-PCR for Patients with Low-Level Viremia.
| HIV-1 2-LTR Circles (copies/106 PBMCs ± SE) | ND4 Mitochondrial DNA (copies/106 PBMCs) a | ||||||
|---|---|---|---|---|---|---|---|
|
|
|
||||||
| Sample | Plasma Viral Load | RT-PCR | WD/Tb | ddPCR | WD/Ta | RT-PCR | ddPCR |
| H (PLLV) | 203 | 3.3 ± 0.8 | 2/3 | 3.3 ± 0.2 | 2/2 | 2.9×107 | 2.3×107 |
| I (VC) | 547 | 2.3 ± 2.2 | 2/3 | 63.7 ± 60 | 2/2 | 3.1×107 | 5.4×107 |
| J (VC) | 780 | 1.0 | 1/3 | 9.4 | 1/2 | 5.3×107 | 3.0×107 |
Abbreviations: PLLV, persistent low-level viremia on ART; VC, viremic controller; RT, real-time; SE, standard error
a
Human mitochondria copy numbers differ between cell and patient and is therefore used to check efficiency of DNA extraction
b
WD/T = number of wells detected/tested