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. Author manuscript; available in PMC: 2012 Dec 9.
Published in final edited form as: Methods Mol Biol. 2008;435:95–108. doi: 10.1007/978-1-59745-232-8_7

Fig. 1.

Fig. 1

T2/onc. T2/onc has SA/polyadenylation (pA) sequences in both orientations to generate loss-of-function mutations in genes in which it lands. Between the two SA are sequences from the long terminal repeat of the MSCV LTR, which contains promoter/enhancer elements to promote overexpression of genes near which it lands. Also present is a splice donor (SD) so that transcripts initiated in the MSCV LTR can splice into downstream endogenous exons.