10× PCR buffer	5 μL
50 mM MgCl2	2 μL
10 mM dNTPs	1 μL
Primary IRDR primer (long IRDR [L2] for left, long IRDR [R] for right)	0.5 μL
Linker primer	0.5 μL
Platinum Taq	0.25 μL
PCR grade water	37.75 μL
Purified, linker-ligated DNA	3 μL