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. Author manuscript; available in PMC: 2012 Dec 9.
Published in final edited form as: Methods Mol Biol. 2008;435:95–108. doi: 10.1007/978-1-59745-232-8_7
10× PCR buffer 10 μL
50 mM MgCl2 4 μL
10 mM dNTPs 2 μL
Nested IRDR primer (new L1 for left, KJC1 for right) 1 μL
Linker nested primer 1 μL
Platinum Taq 0.5 μL
PCR grade water 75.5 μL
Diluted primary PCR product 6 μL