Fig. 2.

Coomassie-blue-stained non-denaturing polyacrylamide gel demonstrating SpoIIE(590–827) phosphatase activity. SpoIIE(590–827) (50 ng) and SpoIIAA∼P (50 μg) were mixed (a molar ratio of 1:2000), incubated for the times indicated below, and the samples were loaded onto a 7.5% polyacrylamide gel. Electrophoresis was performed at 4 °C for 140 min at 100 V. Lanes 1–7: incubation times of 15, 30, 60, 90, 120, 150 and 180 min . Lane 8, 20 μg SpoIIE(590–827); lane 9, 17 μg SpoIIAA∼ P; lane 10, 17 μg SpoIIAA.